The second college that was set up is the NUS College in Bio Valley. Started in June 2002 and located in Philadelphia, NCBV is designed for students to take advantage of the unique opportunities within Greater Philadelphia, for both hi-tech and biotech areas. The region is also home to some of the nation's most prominent medical research facilities including the University of Pennsylvania where students take entrepreneurship related and discipline based courses.

The pioneer group of 14 students departed for the NCBV programme in January 2003 and at a steady state, NCBV will be accepting a total of 30 students per year.